New Extremophilic Lipases and Esterases from Metagenomics
نویسندگان
چکیده
منابع مشابه
New Extremophilic Lipases and Esterases from Metagenomics
Lipolytic enzymes catalyze the hydrolysis of ester bonds in the presence of water. In media with low water content or in organic solvents, they can catalyze synthetic reactions such as esterification and transesterification. Lipases and esterases, in particular those from extremophilic origin, are robust enzymes, functional under the harsh conditions of industrial processes owing to their inher...
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The esterases and lipases from the α/β hydrolase superfamily exhibit an enormous sequence diversity, fold plasticity, and activities. Here, we present the comprehensive sequence and biochemical analyses of seven distinct esterases and lipases from the metagenome of Lake Arreo, an evaporite karstic lake in Spain (42°46'N, 2°59'W; altitude, 655 m). Together with oligonucleotide usage patterns and...
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Improving the health beneficial fatty acid content of meat and milk is a major challenge requiring an increased understanding of rumen lipid metabolism. In this study, we isolated and characterized rumen bacterial lipases/esterases using functional metagenomics. Metagenomic libraries were constructed from DNA extracted from strained rumen fluid (SRF), solid-attached bacteria (SAB) and liquid-as...
متن کاملGel Filtration Applied to the Study of Lipases and Other Esterases.
1. Sephadex G-100 and G-200 gel-filtration columns were calibrated for molecular-weight estimation with proteins of known molecular weights, and used to study the composition of several lipase or esterase preparations. 2. Enzymes from cow's milk, rat adipose tissue and pig pancreas were detected in the column effluents by their ability to liberate free acid from emulsified tributyrin at pH 8.5....
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Dedicated to Prof. Rolf D. Schmid on the occasion of his 60 th birthday [**] We thank Prof. R. discussions and Sandra Vorlová (ITB) for providing recombinant hAChE and bAChE.
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ژورنال
عنوان ژورنال: Current Protein & Peptide Science
سال: 2014
ISSN: 1389-2037
DOI: 10.2174/1389203715666140228153801